Journal: BMC Pulmonary Medicine

Article Title: Astragaloside IV restrains pyroptosis and fibrotic development of pulmonary artery smooth muscle cells to ameliorate pulmonary artery hypertension through the PHD2/HIF1α signaling pathway

doi: 10.1186/s12890-023-02660-9

Figure Lengend Snippet: AS-IV reduced pyroptosis and fibrosis development in hypoxia-treated PASMCs. PASMCs were divided into 3 groups: Nor, Hyp and AS, cultured with normoxia or hypoxia, and treated with or without AS-IV. A - D , Cropped blots of GSDMD-N, NLRP3, cleaved Caspase-1, Fibronectin and Collagen1 and quantification of grayscale value (the samples derived from the same experiment and that gels/blots were processed in parallel). E , IL-1β and IL-18 levels in cell supernatant were measured by ELISA. F , Cell death was detected by PI/Hoechst double fluorescent staining. Scale bar = 50 μm. G, LDH activity was detected by a LDH release agent. H - I , Proteins related to extracellular matrix metabolism, including MMP2/9 and TIMP4 were detected by ELISA method. * P <0.05, ** P <0.01, *** P <0.001, comparison with Nor group. # P <0.05, ## P <0.01, ### P <0.001, comparison with Hyp group

Article Snippet: The nuclei were counterstained with Hoechst 33,258 (Abcam, #ab228550), and the image was visualized under a microscope and analyzed by the image J software.

Techniques: Cell Culture, Derivative Assay, Enzyme-linked Immunosorbent Assay, Staining, Activity Assay, Comparison

Journal: BMC Pulmonary Medicine

Article Title: Astragaloside IV restrains pyroptosis and fibrotic development of pulmonary artery smooth muscle cells to ameliorate pulmonary artery hypertension through the PHD2/HIF1α signaling pathway

doi: 10.1186/s12890-023-02660-9

Figure Lengend Snippet: AS-IV depressed pyroptosis and the production of fibrosis markers induced by hypoxia in PASMCs through the PHD2-HIF1α axis. A - D , PASMCs were divided into 5 groups: Nor, Hyp, AS, AS + si-PHD2 and LW6. Cropped blots of GSDMD-N, NLRP3, cleaved Caspase-1, Fibronectin and Collagen1 (the samples derived from the same experiment and that gels/blots were processed in parallel), and quantitation of the pyroptosis markers and the fibrosis markers. E , Cell death was detected by PI/Hoechst double fluorescent staining. Scale bar = 50 μm. F , IL-1β and IL-18 levels in cell supernatant were measured by ELISA. G, LDH activity was detected by a LDH release agent. H - I , Proteins of MMP2/9 and TIMP4 were detected by ELISA method. * P <0.05, ** P <0.01, *** P <0.001, comparison with Nor group. # P <0.05, ## P <0.01, ### P <0.001, comparison with Hyp group. ^ P <0.05, ^^ P <0.01, ^^^ P <0.001, comparison with AS group

Article Snippet: The nuclei were counterstained with Hoechst 33,258 (Abcam, #ab228550), and the image was visualized under a microscope and analyzed by the image J software.

Techniques: Derivative Assay, Quantitation Assay, Staining, Enzyme-linked Immunosorbent Assay, Activity Assay, Comparison