Journal: bioRxiv

Article Title: RGG peptide induces the disassembly of disease-relevant FUS and TDP43 condensates

doi: 10.1101/2025.03.19.643735

Figure Lengend Snippet: (A) Graph representing the distribution of FUS-P525L protein in nucleus and cytoplasm. The fluorescent intensities of the nucleus and cytoplasm were calculated from the experiment as performed in , and the ratio is plotted here. A student-paired t-test was used to calculate the significance value (n=6). (B) Incucyte images (real-time cell death analysis) representing the cellular uptake of propidium iodide (PI) in different conditions in HeLa cells. Scale bar=100um. (C) Graph representing the number of propidium iodide (PI) positive cells (dead cells) from the experiment as performed in B. The time on the x-axis reflects the time-point after transfection in hours. The significance was calculated using 2-way ANOVA with multiple comparisons (n=4). Error bars in all graphs represent mean ±SEM, and the same color points in A depict the data from a single experimental set. *, **, ***, and **** denote p-value≤0.05, ≤0.01, ≤0.001, and ≤0.0001, respectively.

Article Snippet: The cell death analysis was performed using the IncuCyte S3 live-cell analysis instrument (Sartorius), and the change in the number of PI-positive cells (dead cells) in different conditions was plotted in the graph.

Techniques: Transfection

Journal: bioRxiv

Article Title: RGG peptide induces the disassembly of disease-relevant FUS and TDP43 condensates

doi: 10.1101/2025.03.19.643735

Figure Lengend Snippet: Incucyte images representing the cellular uptake of propidium iodide (PI) in different conditions in HeLa cells. Scale bar=100um. The images are part of and .

Article Snippet: The cell death analysis was performed using the IncuCyte S3 live-cell analysis instrument (Sartorius), and the change in the number of PI-positive cells (dead cells) in different conditions was plotted in the graph.

Techniques:

Journal: Cancer Cell International

Article Title: PIBF1 (p.R405Q) germline variant identified in cancer susceptibility family impairs protein stability and function

doi: 10.1186/s12935-025-04132-y

Figure Lengend Snippet: PIBF1 (p.R405Q) mutation is a candidate gene for hereditary cancer syndromes. A . The expression of PIBF1 is low in BRCA patients in the UALCAN database. B . The expression level of PIBF1 in the UALCAN database is negatively correlated with the tumor grade. C . The Kaplan-Meier Plotter plots survival curves of PIBF1 and low expression of PIBF1 was correlated with poor prognosis of breast cancer. D , E . Q-PCR and WB detection of PIBF1-WT and PIBF1(p.R405Q) in the MDA-MB-231 and MDA-MB-468. F . CCK8 was performed to detect the effect of PIBF1-WT and PIBF1(p.R405Q) on the cell growth of breast cancer cells. G , H . Plate cloning assay to detect the effect of PIBF1-WT and PIBF1(p.R405Q) on the proliferation of breast cancer cells. I . Transwell assay to detect the effect of PIBF1-WT and PIBF1(p.R405Q) on the invasion ability of breast cancer cells. J . WB assay to detect the regulatory effect of PIBF1-WT and PIBF1(p.R405Q) on ERK phosphorylation. All data are presented as mean ± SEM ( n = 3 independent cell culture experiments), *, P < 0.05; **, P < 0.01; ***, P < 0.001

Article Snippet: The medium was refreshed every 24 h with a 1:10 dilution of CCK8 reagent (Cat#C0005, TargetMol, USA) and incubated at 37 °C for 3 hours before measuring absorbance at 450 nm using a microplate reader (EnSpire 2300, PerkinElmer, USA).

Techniques: Mutagenesis, Expressing, Cloning, Transwell Assay, Phospho-proteomics, Cell Culture